LEUKEMIA INHIBITORY FACTOR HUMAN

Code: SRP9001-10UG D2-231

Application

One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated st...


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€389.00 10UG
€478.47 inc. VAT

Application

One of the main uses of LIF in research is in mouse embryonic stem cell culture maintenance. LIF is necessary to maintain the stem cells in an undifferentiated state while retaining their proliferative potential or pluripotency. Removal of LIF pushes stem cells toward differentiation.

Leukemia inhibitory factor (LIF) has been used to evaluate its effect on embryo development and aneuploidy in matured (IVM) human oocytes, in vitro.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts. It also inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B cell lymphoma) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress. LIF is capable of maintaining the totipotency of embryonic stem cells. It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.

General description

Leukemia Inhibitory Factor (LIF) gene is mapped on the human chromosome at 22q12.2.

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines. This protein was originally identified as a protein that prevented the growth of a leukemic cell line.

LIF is manufactured using an all-human production system, with no serum.

Physical form

This product is supplied as a solution in 0.2 µm filtered phosphate buffered saline with no additives or carrier proteins. It is aseptically filled.

Preparation Note

Briefly centrifuge the vial before opening. After initial thawing it is recommended to store the protein in working aliquots at -20°C. The product can be diluted in PBS.

assay≥95% (SDS-PAGE)
biological sourcehuman
formliquid
Gene Informationhuman ... LIF(3976)
impurities≤1 EU/µg protein Endotoxin level
mol wt20 kDa
packagingpkg of 10 µg
potency≤0.5 ng/mL M1 cells proliferation inhibition EC50
Quality Level200
recombinantexpressed in HEK 293 cells
storage temp.−20°C
technique(s)cell culture | mammalian: suitable
UniProt accession no.P15018
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